01984nas a2200229   4500008004100000245010600041210006900147300001100216490000700227520109300234653002601327653003101353653002101384653002701405653002601432100001401458700001901472700001601491700001601507700001701523856021401540       2009                        eng d00aHighly polymorphic microsatellites in the North American snakeweed grasshopper, Hesperotettix viridis0 aHighly polymorphic microsatellites in the North American snakewe  a19 -210 v183 a<p>Microsatellite markers are preferred for fine-scale population genetic studies requiring high resolution. The grasshopper Hesperotettix viridis (Thomas) is an oligophagous species that feeds on composites and often exhibits locally restricted diets. Divergence in host plant use in some localities is seen where co-occurring subpopulations select alternate plant species, as expected with the evolution of host shifts and associated lineage divergence. To characterize the host-associated divergence patterns among populations of H. viridis, we developed markers from two microsatellite-enriched genomic libraries. Here we report the characterization and optimization of seven polymorphic di- and tri-nucleotide microsatellite loci for this species. One hundred and six individuals from 5 populations were tested for polymorphism. The number of alleles varied from 4 to 38 in all the populations. Ho ranged from 0.339 to 0.790. Homozygote excess was observed across loci, perhaps due to inbreeding. This is the first report of microsatellite markers for the subfamily Melanoplinae.</p>10aHesperotettix viridis10ahost-associated divergence10alocal adaptation10amicrosatellite markers10asnakeweed grasshopper1 aGrace, T.1 aJoern, Anthony1 aBrown, S.J.1 aApple, J.L.1 aWisely, S.M.  uhttps://bioone.org/journals/Journal-of-Orthoptera-Research/volume-18/issue-1/034.018.0111/Highly-Polymorphic-Microsatellites-in-the-North-American-Snakeweed-Grasshopper-iHesperotettix/10.1665/034.018.0111.full