SEA01 Soil extracellular enzyme activity data from the Belowground Plot Experiment

Summary

Short name: 

SEA01

Abstract: 

Data describe the activity of soil extracellular enzymes collected approximately every month in 2015 from the Belowground Plot Experiment. The measured enzymes depolymerize soil organic matter to release labile carbon, nitrogen, and phosphorus. Soil carbon and nitrogen were measured in July 2015 only, since these soil variables are not expected to change monthly.

DOI: 10.6073/pasta/caff8cde33a6dca2b007e85b5c4a4000

Published on EDI/LTER Data Portal

Citation Suggestion: 

Nieland, M., Zeglin, L. . 2023. SEA01 Soil extracellular enzyme activity data from the Belowground Plot Experiment . Environmental Data Initiative. http://dx.doi.org/10.6073/pasta/caff8cde33a6dca2b007e85b5c4a4000.

Data set ID: 

176

EML revision ID: 

1

Data sources: 

SEA011.csv

Variables: 

DataCode
  • Label:
  • Definition: Dataset code
  • Type: Nominal
  • Missing values: None specified
RecType
  • Label:
  • Definition: Record type
  • Type: Physical quantity
  • Unit: dimensionless
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
Recyear
  • Label:
  • Definition: Year of sample
  • Type: Physical quantity
  • Unit: Year(YYYY)
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
RecMonth
  • Label:
  • Definition: Month of sample collection
  • Type: Nominal
  • Missing values: None specified
Plot
  • Label:
  • Definition: Field plot IDs
  • Type: Nominal
  • Missing values: None specified
Burn
  • Label:
  • Definition: Burned (B) or unburned (UB)
  • Type: Nominal
  • Missing values: None specified
Nutrient
  • Label:
  • Definition: Fertilization treatment
  • Type: Code list
  • Codes:
    • C = control
    • N = nitrogen
    • P = phosphorus
    • NP = nitrogen and phosphorus
  • Missing values: None specified
BG
  • Label:
  • Definition: Betaglucosidase activity (nmol substrate / g dry soil / hr)
  • Type: Physical quantity
  • Unit: nanoMolesPerGramPerHour
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured
CBH
  • Label:
  • Definition: Cellobiohydrolase activity (nmol substrate / g dry soil / hr)
  • Type: Physical quantity
  • Unit: nanoMolesPerGramPerHour
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured
NAG
  • Label:
  • Definition: N-acetylglucosaminidase activity (nmol substrate / g dry soil / hr)
  • Type: Physical quantity
  • Unit: nanoMolesPerGramPerHour
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured
LAP
  • Label:
  • Definition: Leucyl aminopeptidase activity (nmol substrate / g dry soil / hr)
  • Type: Physical quantity
  • Unit: nanoMolesPerGramPerHour
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured
PHOS
  • Label:
  • Definition: Phosphatase activity (nmol substrate / g dry soil / hr)
  • Type: Physical quantity
  • Unit: nanoMolesPerGramPerHour
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured
PHX
  • Label:
  • Definition: Phenol oxidase activity (nmol substrate / g dry soil / hr)
  • Type: Physical quantity
  • Unit: nanoMolesPerGramPerHour
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured
GWC
  • Label:
  • Definition: Gravimetric water content (g water / g dry soil)
  • Type: Physical quantity
  • Unit: gramsPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured
PercentC
  • Label:
  • Definition: Percent carbon in soil
  • Type: Physical quantity
  • Unit: percent
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured
PercentN
  • Label:
  • Definition: Percent nitrogen in soil
  • Type: Physical quantity
  • Unit: percent
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = Missing or Not Measured

Categories

Core Areas: 

Konza Keywords: 

Dates

Date Range: 

Monday, January 5, 2015 to Thursday, December 31, 2015

Publication Date: 

Thursday, May 18, 2023
People

Owner/Creator: 

Matthew Nieland
Dr. Lydia Zeglin

Contact: 

Konza LTER

Additional Project roles: 

Victoria Floyd

Role: 

Associated Researcher
Christine Carson

Role: 

Associated Researcher

Metadata provider: 

Konza LTER
Related

Related publications: 

Product-inhibition feedbacks, not microbial population level tradeoffs or soil pH, regulate decomposition potential under nutrient eutrophication
Downloads
Methodology

Methods: 

Research purpose: To assess enzyme-mediated feedbacks to increases in nutrient availability from long-term fertilization.
Location of sampling: Belowground Plot Experiment
Frequency of sampling: Monthly for soil extracellular enzyme activities.
variable measured: Activities of extracellular enzymes: Beta-glucosidase, cellobiohydrolase, N-acetylglucoaminidase, leucyl aminopeptidase, acid phosphatase, and phenol oxidase. Soil chemistry: Total carbon and nitrogen and gravimetric water content.
laboratory methods: Soil samples from each plot were collected as composites of four 2 cm diamter, 15 cm deep soil cores.
form of data output: Extracellular enzyme activities were measured using short-term, room temperature assays with fluorometric organic substrates (Saiya-Cork et al. 2002, SBB 34(9): 1309-1315.; Zeglin et al. 2013, Ecology 94(10): 2334-2345.). Total carbon and nitrogen were quantified using thermal conductivity. Gravimetric water content was measured by drying soil samples overnight at 105 Celsius and weighing water mass loss.P
quality assurance: -999 or NA were used for quality control / quality assurance.
instrumentation: FilterMax F5 Multimode Microplate Reader; Flash EA 1112 NC Analyzer

Maintenance: 

complete